Input
0 characters
Exon Junction Markers (Optional)
(e.g., if clipped, enter starting position)
💡 Enter positions from the original reference. They'll be auto-adjusted to match your sequence.
Sequence Visualization
Sequence will appear here after analysis.
Primer Table
| Name | Sequence | Length (bp) | GC% | Tm (°C) | ΔG 5' / 3' | Orientation | Hairpin | Adjust Position | Analyze |
|---|
Primer Cross-Dimer Analysis
| Primer Pair | Binding Details |
|---|